International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Analysis Study Research Paper | Pharmaceutical Science | India | Volume 11 Issue 8, August 2022


Reversed Phase HPLC Method Development and Simultaneous Determination of Topical Dosage Form

Puja Vyawhare | Rupali Tasgaonkar


Abstract: A novel, rapid, isocratic, reversed phase HPLC method was developed for simultaneous determination of betamethasone dipropionate, miconazole nitrate, chlorocresol in topical dosage form. The separation was carried out on C18 prontosil (250 x 4.6mm x 5?) column. The mobile phase used for this study consists of acetonitrile and phosphate buffer of pH 2 in the ratio of 70: 30 v/v respectively. The flow rate was kept as 1 mL per minute. In this method, UV detector was used with wavelength 235 nm. The retention time of chlorocresol, miconazole nitrate, betamethasone dipropionate was 4.30 min, 5.13 min, 6.49 min, respectively. Linearity was observed for betamethasone dipropionate, miconazole nitrate, chlorocresol in concentration range of 1 to 3 ?g/mL, 40 to 120 ?g/mL, 2 to 6 ?g/mL respectively.


Keywords: High performance liquid chromatography, Simultaneous determination, Betamethasone dipropionate, Miconazole nitrate, Chlorocresol


Edition: Volume 11 Issue 8, August 2022,


Pages: 39 - 43


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