Development and Validation of RP-HPLC Method for Estimation of a Multidrug Marketed Formulation

Damini Jaiswal, Avinash V. Chandewar

Abstract: Phenylephrine HCL, Cetirizine HCL and Nimesulide, one of the Nasal decongestant, Antihistaminic, Non-Steroidal Anti-inflammatory, Respectively. In this regard the research& development of reliable analytical methods for self -determination. It is soluble in acetonitrile & insoluble in acetone. The main aim of present research work is to develop & validate a method for estimation of cetirizine HCL, Phenylephrine HCL, & Nimesulide by using UV- visible spectrometry and High-Performance liquid chromatography. RP-HPLC method for estimation of pharmaceutical dosage form was developed successfully. Chromatographic separation was performed on Phenomenex GeminiC18 150X4.60 mm 5?mParticle size. The mobile phase consisting of a Acetonitrile: water PH 3.28. 50:50 was delivered at arate1ml /min. The detection was made 215 nm. Mobile phase was degassed before use & separation was done.

Keywords: PhenomenexGemini 150x4.60 mm, RP-HPLC, Acetonitrile, PH 3.28 215 wavelength

How to Cite?: Damini Jaiswal, Avinash V. Chandewar, "Development and Validation of RP-HPLC Method for Estimation of a Multidrug Marketed Formulation", Volume 12 Issue 3, March 2023, International Journal of Science and Research (IJSR), Pages: 590-596, https://www.ijsr.net/getabstract.php?paperid=SR221222171133, DOI: https://dx.doi.org/10.21275/SR221222171133