Enzymatic Properties and Optimization of Immobilized Lipase from Aspergillus niger by using the ZIF-8 as a Carrier

Naeema Matuoog, Latifa Abdel-Hafid Jwieli, Sanaa ALshawish, Ibtisam M. Ahmadi

Abstract: Enzymes are biocompatible and biodegradable molecules, The esoporous hierarchical (ZIF-8) was synthesized in an aqueous solution and prepared using a modulating agent (TEA) to obtaining a hexahedral shape. The (CTAB) was added as a morphological regulating agent. The results of (PXRD) was showed that the morphological properties of ZIF-8 were modified because of the encapsulation. The ANL was encapsulated in ZIF-8, the immobilization efficiencies exceeded 91% and the activity recovery of reached 2150% under the optimized conditions. In terms of enzymatic properties, the results revealed that the temperature and pH stability of the immobilized enzyme increased compared with free lipase.

Keywords: Enzymatic Properties, Aspergillus niger lipase, ANL, ZIF-8, Immobilization, Esterification, Biotechnology, lipases, Zeoliticimidazolate framework, Immobilization efficiency, lipase activity recovery