Analytical Method Development and Validation for the Determination of Isoniazid in, Isoniazid and Ethambutol Hydrochloride Tablets

Viswanatha Reddy, Vishal Shinde, Anjali A. Rajbhoj, Suresh T. Gaikwad

Abstract: A simple method using HPLC system for the quantification of Isoniazid, in a fixed-dose combination (FDC) of anti-tuberculosis drug product was developed and validated. The chromatographic separation of Isoniazid (INH) was carried out using Waters Symmetry C8 column (150× 4.6 mm: Particle size: 5 μm), UV detection at 262 nm, and an isocratic system with composed of mobile phase of Phosphate buffer solution (pH 6.8) and Acetonitrile in the ratio of 980: 20, at a flow rate of 1.0 mL/min. The retention time of Isoniazid was about 3.8 minutes.

Keywords: Antituberculosis, Isoniazid, Ethambutol Hydrochloride, HPLC method

How to Cite?: Viswanatha Reddy, Vishal Shinde, Anjali A. Rajbhoj, Suresh T. Gaikwad, "Analytical Method Development and Validation for the Determination of Isoniazid in, Isoniazid and Ethambutol Hydrochloride Tablets", Volume 9 Issue 7, July 2020, International Journal of Science and Research (IJSR), Pages: 1876-1880, https://www.ijsr.net/getabstract.php?paperid=SR20728070058, DOI: https://dx.doi.org/10.21275/SR20728070058