Structure of Sporisorium Scitamineum Isolates, the Causative Agent of Sugarcane Smut in Cote Divoire

Yao Kouadio Jacques-Edouard, Kouame Konan Didier, Kouame Koffi Gaston, Kassi Koffi Fernand Jean-Martial, N'guessan Aya Carine, Eponon Eboa Christophe Ghislain, Kone Daouda

Abstract: The aim of this work was to assess the macroscopic and microscopic characteristics and the variability between isolates of Sporisorium scitamineum present in C?te d'Ivoire. Smut whip samples were collected in 2017 and 2018 in C?te d'Ivoire from four agro-industrial complexes. The fungus was isolated out on PDA medium at the Laboratory. The morphological characters were described on the basis of the growth diameter, texture and colonies colors. The microscopic description focused on the spore's size from medium and teliospores from whips. An analysis of variance was applied to the measured parameters and the means comparison was performed according to the Newman-Keuls test (post-hoc ANOVA test) at the significance level of 5% using the STATISTICA 7.1 software. Macroscopic results indicated two groups of isolates. Group 1 contains 85% of the isolates and characterized by a white mycelial colony with a cottony appearance. The G2 group comprising 15% of the isolates and marked by a yeast-shaped mycelial colony. The diameter of the teliospores varied between 8.36 ?m and 10.25 ?m. The size of the spores varied between 9.14 and 11.65 ?m. This study made it possible to highlight the variability between the isolates of Sporisorium scitamineum in C?te d'Ivoire.

Keywords: Smut disease, Structures of isolates, Sporisorium scitamineum, Sugarcane, Ivory Coast