HPLC, Antioxidant and Antibacterial Activities of Methanolic Extract of Berberis aristata Stem
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 109 | Views: 297

Research Paper | Chemistry | India | Volume 3 Issue 11, November 2014 | Popularity: 6.7 / 10


     

HPLC, Antioxidant and Antibacterial Activities of Methanolic Extract of Berberis aristata Stem

Ovas Dar, Reena Lawrence, Sikander Dar


Abstract: The components in methanolic extract of Berberis aristata stem were identified by HPLC. The three components which were identified are Berberine (95.52 %), Palmitine (2.64 %) and Jatrorrhizine (1.83 %). The antioxidant activity of the methanolic extract were determined by DPPH (1, 1-Diphenyl-2-picryl hydrazyl) assay and Nitric oxide scavenging method. The methanolic extract was able to reduce stable purple colored DPPH reaching 50 % of reduction with IC50 values as IC50 for methanolic extract=0.90 mg/ml, IC50 for Gallic acid = 0.61 mg/ ml IC50 for BHT = 0.41 mg/ml. The methanolic extract reduced the concentration of DPPH free radical with an efficacy near to that standard Gallic acid. In case of Nitric oxide scavenging assay, IC50 values have been found to be 0.80 mg/ml, 0.65 mg/ml and 0.60mg/ml for the methanolic extract, gallic acid and BHT respectively. The antibacterial activity of methanolic extract was evaluated on bacteria strains like Escherichia coli, Staphylococcus aureus and Bacillus subtilis by using agar well diffusion method. The results were compared with ampicillin, a commercial antibacterial agent. Methanolic extract showed maximum (19mm) zone of inhibition against Bacillus subtilis. The MIC values of methanolic extract for different pathogenic bacteria ranged from 37 to 111g/ml.


Keywords: Methanolic extract, HPLC, IC50, Zone of inhibition, MIC


Edition: Volume 3 Issue 11, November 2014


Pages: 1137 - 1141



Make Sure to Disable the Pop-Up Blocker of Web Browser


Text copied to Clipboard!
Ovas Dar, Reena Lawrence, Sikander Dar, "HPLC, Antioxidant and Antibacterial Activities of Methanolic Extract of Berberis aristata Stem", International Journal of Science and Research (IJSR), Volume 3 Issue 11, November 2014, pp. 1137-1141, https://www.ijsr.net/getabstract.php?paperid=OCT141038, DOI: https://www.doi.org/10.21275/OCT141038

Similar Articles

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Chemistry, Congo (DRC), Volume 11 Issue 5, May 2022

Pages: 1417 - 1420

Chemical Composition of the Leaves of Tristemma mauritianum Consumed by the Population of Kisangani (DR Congo)

Celestin BAMAWA, Francois FOMA

Share this Article

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Chemistry, India, Volume 11 Issue 5, May 2022

Pages: 1689 - 1692

Analytical Method Development and Validation for Larcenidepin and its Stages by High Performance Liquid Chromatography

Virendra Sopan Pathare

Share this Article

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Chemistry, India, Volume 13 Issue 5, May 2024

Pages: 993 - 998

Evaluation of Preliminary Parameter and Antimicrobial Potential of Pterocarpus Santalinus

Pooja Dhadse, Jyoti Saxena

Share this Article

Downloads: 2 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Chemistry, India, Volume 11 Issue 5, May 2022

Pages: 1852 - 1855

Analytical Method Development and Validation for Baclofen and its Stages by High Performance Liquid Chromatography

Pathare Virendra Sopan

Share this Article

Downloads: 4 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Chemistry, India, Volume 12 Issue 7, July 2023

Pages: 2180 - 2184

Comprehensive Analysis of Antioxidant and Antimicrobial Properties of Aegle Marmelos Leaf Extracts

Laxmi Chaudhari, Dr. Dipali Gulwade

Share this Article
Top