Role of Basal Media, Carbon Sources and Growth Regulators in Micropropagation of Two Valuable Medicinal Orchids of Bangladesh

Mohammed Mozammel Hoque, Mohammad Al-Forka

Abstract: An efficient, protocol for in vitro germination seeds of and micropropagation of two the valuable indigenous medicine Orchids namely, Bulbophyllum lilacinum Ridl. and Cymbidium aloifoloum (L) Sw. were developed by enhanced shoot multiplication, nodal and leaf segments was designed. The medium type and various carbon sources, plant growth regulators markedly influenced in vitro propagation of B. lilacinum Ridl. and C. aloifoloum (L) Sw. The in vitro protocorms production system was investigated on three 0.8 % (w/v) agar solidified basal media namely, Phytomax Sigma Chemical Co. (PM), Modified Vacin and Went (MVW), Murashige and Shook, 1962 (MS) medium with the synergistic combination of Auxin (IAA 1.0-2.0 mgl-1, IBA 2 mgl-1, NAA 1.0-2.0mgl-1, 2, 4-D 1.0mgl-1), Cytokinins (BAP 0.5-2.0mgl-1, Kn 0.5-2mgl-1) and 2 % -3 % sucrose which induced maximum number of shoots as well as beneficial shoot length. Sub culturing of nodal segments and leaf segments on similar medium enabled continuous production of healthy shoots with similar frequency. Rooting was highest (2.96 cm) on half strength MS medium containing 1.5 % sucrose. The in vitro growing seedling were then transferred to outside natural environment through successive phases of acclimatization.

Keywords: Micropropagation, Protocorms, Bulbophyllum lilacinum, Cymbidium aloifoloum, Shoot multiplication and Growth regulators