Detection of Antiplatelets Antibody Specially GPIIb/IIIa using MIAPA Method in Baghdad Patients with ITP

Baan A. Mtashar, Zeyad A. Shabeeb, Zainab F. Ashoor

Abstract: Immune thrombocytopenic purpura (ITP), is an autoimmune disease that caused by sensitization of platelets by auto-antibodies leading to increase platelet destruction mainly by spleen macrophages. Methods This study has determined and characterized the antiplatelet glycoproteins in Adult patients with ITP. Fifty patients, who were hospitalized with clinical signs of ITP in national center of hematology during 6 months, went under our clinical studies in a research project. ELISA and MAIPA (Monoclonal Antibody Immobilization of Platelet Antigens) methods were used to determine serum anti-platelet antibodies level. Results The anti-platelet antibodies level above mean + 3SD of control group was assumed as positive. The platelet counts ranged between 0 109/L and 115 109/L. Among the patients 52 % of them were antiplatelet antibodies positive with ELISA method. . Antibody against platelet GPIIb/IIIa using MAIPA method were 28 %, Conclusion MAIPA is the specific method for the detection of antibody against glycoprotein antigens, it has the advantage of differentiating between immune and non-immune thrombocytopenia.

Keywords: Anti-platelet antibody, gpIIb/IIIa, ELIZA, MAIPA