Antioxidant Potential of Selected Medicinal Plants of Trans- Himalayan Region

Suman Tiga, Prabhu Sarangi, Omprakash Chaurasia, Bhuvnesh Kumar

Abstract: The objective behind this study was to determine and compare the antioxidant levels and activities of selected medicinal plants of Trans -Himalayan region. Considering the growing interest in possessing the antioxidant capacity of medicinal plants, an effort has been carried to recognize the effect of antioxidants in different plants by using DPPH (1, 1?diphenyl-2-picrylhydrazyl) radical assay, ABTS (2, 2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assay, FRAP (ferric reducing antioxidant power), total phenolic content (TPC), total flavnol, total proanthoanthocynidin and total flavonoid content. Extraction of antioxidant compound from plants part was done with 80% methanol. The frems of inhibitory concentration (IC50) in DPPH ranged from 8.604 to 2.306 ?g/mg. (IC50) in ABTS ranged from 1.38 to 7 ?g/mg and FRAP ranged from (102.66 to 385.76 FeSO4.7H2O0. Highest antioxidant activity was observed in Saussaria lappa. TPC were expressed as gallic acid equivalent (GAE) in ?g per mg weight. Total flavnol, total proanthoanthocynidin and total flavonoid content were expressed as ?g of catechin equivalent per milligram of dry weight Highest polyphenol and flavonoid content was found in Arnebia euchroma (244.58 ?gGAE/mg) and (17.77 ?gCE/mg) respectively. Highest flavnol content was found in Inula racemose (70 ?gCE/mg) and proanthocynidin content was highest in in R. webbanium.

Keywords: Antioxidant potential, Polyphenol, Medicinal plant, Radical scavenger, Trans Himalayan