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Masters Thesis | Surgery | Volume 15 Issue 6, June 2026 | Pages: 18 - 23 | India
Microbial Profile and Antibiotic Sensitivity of Bile in Patients Undergoing Laparoscopic Cholecystectomy: A Prospective Observational Study
Abstract: Bile, traditionally considered sterile, has been shown to harbor microorganisms in patients with gallbladder disease, particularly in acute and chronic cholecystitis. The presence of bactibilia has important clinical implications, including postoperative infections and antibiotic selection. Aim: To evaluate the microbial profile and antibiotic sensitivity patterns of bile in patients undergoing laparoscopic cholecystectomy and to assess correlation with ultrasonographic findings. Materials and Methods: This prospective observational study was conducted on 100 patients undergoing laparoscopic cholecystectomy over a period of 18 months. Intraoperative bile samples were collected aseptically and sent for microbiological culture and antibiotic sensitivity testing. Clinical, radiological, and microbiological data were analyzed. Results: Bile culture positivity was observed in 40% of patients. The highest positivity was seen in acute cholecystitis (65.4%), followed by chronic cholecystitis (43.5%) and cholelithiasis (25.5%). Escherichia coli (45.8%) was the most common organism isolated, followed by Klebsiella species, Pseudomonas species, and Staphylococcus species. Gram-negative organisms showed highest sensitivity to Imipenem and Amikacin and Ceftriaxone while reduced sensitivity was observed for Ciprofloxacin. Conclusion: Bacterial colonization of bile is common in gallbladder disease. Gram-negative organisms predominate, and increasing antibiotic resistance necessitates culture-guided therapy.
Keywords: Bactibilia, Laparoscopic cholecystectomy, Bile culture, Antibiotics Sensitivity, Escherichia coli, Gallbladder disease, Antimicrobial resistance
How to Cite?: Dr. Sachin Patel, Dr. (Lt. Col.) Sangeeta Tiwari, Dr. Rajesh Kumar Agarwal, "Microbial Profile and Antibiotic Sensitivity of Bile in Patients Undergoing Laparoscopic Cholecystectomy: A Prospective Observational Study", Volume 15 Issue 6, June 2026, International Journal of Science and Research (IJSR), Pages: 18-23, https://www.ijsr.net/getabstract.php?paperid=MR26411090649, DOI: https://dx.dx.doi.org/10.21275/MR26411090649