Bacteriological Profile and Antibiotic Susceptibility Pattern of Bloodstream Infection Isolates in a Tertiary Care Hospital

Dr. Jyotsna Padhan

Abstract: Background: Bloodstream infections (BSIs) remain a major cause of morbidity and mortality worldwide. Early identification of causative organisms and their antimicrobial susceptibility patterns is crucial for appropriate therapy and improved patient outcomes. Objective: To identify the bacterial pathogens causing bloodstream infections and to determine their antibiotic susceptibility patterns in a tertiary care hospital. Methods: A hospital-based cross-sectional study was conducted over six months (January 2024?June 2024) among 874 patients clinically suspected of bloodstream infections admitted to intensive care units and wards of a tertiary care hospital in Bhubaneswar, India. Blood samples were collected aseptically prior to antibiotic administration and processed using conventional blood culture techniques. Isolates were identified by standard microbiological methods. Antimicrobial susceptibility testing was performed using the Kirby?Bauer disk diffusion method as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: Out of 874 blood samples, 134 (15.3%) were culture-positive. Gram-positive cocci constituted 55.9% of isolates, predominantly Staphylococcus aureus (45.5%), followed by coagulase-negative staphylococci (8.2%) and Enterococcus species (2.2%). Gram-negative bacilli accounted for 44.1% of isolates, with Klebsiella species (34.3%) being the most common, followed by Escherichia coli (4.4%), Acinetobacter species (2.9%), and Pseudomonas aeruginosa (2.2%). Gram-positive isolates showed high sensitivity to vancomycin (98%), linezolid (88%), and teicoplanin (88%). Gram-negative isolates were most sensitive to imipenem (79.6%) and meropenem (72.8%). Conclusion: Staphylococcus aureus and Klebsiella species were the predominant pathogens causing bloodstream infections. Carbapenems and glycopeptides remain effective against most Gram-negative and Gram-positive isolates, respectively. Continuous antimicrobial surveillance is essential to guide empirical therapy and combat emerging resistance.

Keywords: bloodstream infections, antimicrobial susceptibility, Staphylococcus aureus, Klebsiella species, tertiary care hospital

How to Cite?: Dr. Jyotsna Padhan, "Bacteriological Profile and Antibiotic Susceptibility Pattern of Bloodstream Infection Isolates in a Tertiary Care Hospital", Volume 14 Issue 12, December 2025, International Journal of Science and Research (IJSR), Pages: 2322-2323, https://www.ijsr.net/getabstract.php?paperid=MR251229125916, DOI: https://dx.doi.org/10.21275/MR251229125916