International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Experimental Result Paper | Pathology | India | Volume 13 Issue 2, February 2024


Basic Staining Methods for Sperm Analysis in Oligospermia - A Study in Tertiary Care Hospital

Dr. Ashwini Natekar | Dr. Krishna Govind | Dr. B. N. Hiwale


Abstract: Approximately 15% of married couples have infertility problems. Twenty percent of them originate from men, 30% - 40% originate from both men and women. So, one half of the infertility couples have male - factor associated infertility. [1] Assessment of sperm morphology is the most important criterion for determining the quality of a semen sample. The goal of identifying an ideal technique for sperm staining is to ease the visualization of cells and provide a better identification of the abnormalities. This is an important factor for successful fertilization and early embryonic development in assisted reproductive techniques. [2] The accuracy of sperm morphology assessment depends on careful smear preparation, fixation and staining since these procedures can affect the sperm dimensions significantly. Many staining methods such as Haematoxylin & Eosin, Giemsa, Papanicolau, Eosin - Nigrosin and Leishman have been used for sperm staining. Some of the commercially available stains such as Shorr, Janus Green and Sperm Blue are too expensive to be routinely used in developing countries like India. [3] A few stains may cause a slight change in the measurement values of spermatozoa because fixatives may shrink the cells a little. [4] This creates a need for an ideal, simple, and cost effective staining method which also does not alter the sperm morphology for an accurate evaluation of the same. Many of the methods used to stain semen results in very distinct coloring of the sperm but unfortunately they do not distinguish their individual structures which play a key role in the fertilization process. The aim of this study was to identify sperm structures using two staining techniques. This staining technique can be used to stain sperm structures that can not be seen in other methods of slide preparation, which means that it can be considered for routine use in assessing the fertility.


Keywords: Infertility, sperm morphology, staining techniques, assisted reproductive techniques, cost effectiveness


Edition: Volume 13 Issue 2, February 2024,


Pages: 829 - 832


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