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Research Paper | Pharmacology and Toxicology | India | Volume 13 Issue 2, February 2024
In Vivo Study of Unani Herbal Formulation Beikh-E-Karafs (Apium graveolens) Revandchini (Rheum Emodi) Namak-E-Turb (Raphanus sativus) with Lithogenic diet (Cholesterol Cholic acid & Fatty diet) Induced Hasat-ul-Mirarah (Cholelithiasis) in Mice
Dr. Nazima Sultana | Dr. Zaibunnisa Begum
Abstract: Cholelithiasis is a chronic and recurrent hepato-biliary disease resulting from the impaired metabolism of cholesterol, bilirubin, and bile acids. This condition is characterized by the formation of gallstones in the gallbladder and common bile duct. The development of gallstones is a complex process involving various factors such as cholesterol supersaturation, nucleation, and crystal growth. The interaction of genetic, environmental, and lifestyle factors contributes to the pathogenesis of cholelithiasis. Management strategies for cholelithiasis include lifestyle modifications, pharmacological interventions and in some cases, surgical procedures such as cholecystectomy. Understanding the underlying mechanisms of gallstone formation is crucial for the development of effective preventive and therapeutic approaches. The disease was mentioned by Hippocrates as Biliary obstructive disease. Accordingto Unani physicians the Hasath-ul-mirarah is due to imbalanced humours in the body Safra (Bile) and Phlegm are the main factors to produce a "Hasat-ul-mirarah." Aim: "In Vivo study of Unani herbal formulation Beikh-e-Karafs (Apium graveolens) Revandchini (Rheum emodi) Namak-e-Turb (Raphanus sativus) with lithogenic diet (cholesterol, cholic acid & fatty diet) induced Hasat-ul-Mirarah (Cholelithiasis) in Mice". To evaluate the Physiochemical analysis and Standardization of Unani Herbal formulation. To manage the disease and its complication with safe, cost effective and easily available drug in the Unani system of medicine. Material & Methods: Swiss Albino Mice were randomly divided into Control group, Disease Control group, Ethanolic Extract Test Group (Low dose & High dose), Crude Test Group (Low dose & High dose) 6 animals in each group. Gall Stones were induced in mice by feeding lithogenic diet for 8 weeks. After 8 weeks, Disease control group was sacrificed sampled to calculate the incidence of stone formation and biochemical parameters (Serum Cholesterol, triglycerides HDL, SGPT, SGOT, ALP, Bilirubin, Albumin and Total proteins, Serium amylase) and Histopathological assessment was done. The test drug was given for 4 weeks to Ethanolic Extract Test Group (Low dose & High dose) Crude Test Group (Low dose & High dose), after 4week animals was sacrificed biochemical parameters and Histopathological assessment was done. Result: There is significant decreased in biochemical parameters (P<0.05***) & histopathological changes were reduced in liver and the inflammatory changes were less in gall bladder in Ethanolic Extract Test Group (High dose) (0.014mg/kg). The results were less in Crude Test Group (Low dose & High dose) Ethanolic Extract Test Group (Low dose) when compared to Ethanolic Extract Test Group (High dose) Conclusion: Unani herbal formulation given in Ethanolic extract form can decrease the incidences of stone formation and improve the biochemical parameters, which may be one of the mechanisms in the treatment and prevention of gall stone formation.
Keywords: Cholelithiasis, Unani herbal formulation, biochemical parameters, ethanolic extract & crude test drug
Edition: Volume 13 Issue 2, February 2024,
Pages: 1551 - 1562