Comparison of Escherichia Coli Bacteria Growth on Soybean Alternative Modification Media

Pestariati, Suhariyadi, Syamsul Arifin

Abstract: Escherichia coli bacterial infections often cause urinary tract infections. Diagnosis of Escherichia coli through examination of microscopic, culture, and serological specimens. Examination of culture of Escherichia coli requires EMB media containing protein. Soybeans contain 30 grams of protein per 100 grams, thus potentially replacing protein in EMB media. This study aims to determine the growth of Escherichia coli in alternative soybean modification media and manufacturer EMB. This study was an experimental laboratory conducted at the Bacteriology Laboratory. Media Alternative modification of soybeans uses a variation of 0%, 25%, 50%, and 100% as a substitute for protein, then each concentration is added lactose 10 grams, dipotasium hydrogen phosphate 2 grams, eosin 2 grams and 15 grams so as compactor, Methylene Blue 0.06 gram as an indicator of fermentation, then planted Escherichia coli in each alternative media of soybean modification and manufacturer's EMB as gold standard 370C 24 hours Analysis of the data was described statistically. The results showed that the alternative media modified soybeans in order to replace 100% EMB (Eosine Methylene Blue) media with an average number of Escherichia coli colonies grown in this medium as many as 261.5 colonies, almost the same as the average colony on EMB media as a Gold standard of 358 colonies

Keywords: Soybeans, Escherichia coli, Eosine Methylene Blue Activity