Downloads: 132 | Views: 383
Research Paper | Biotechnology | Afghanistan | Volume 8 Issue 12, December 2019 | Popularity: 6.7 / 10
Molecular Cloning of Flavone Synthase I Gene from Cumin (Cuminum Cyminum)
Kobra Yousefi, Farzaneh Razmju, Aziz Ahmad Osmani
Abstract: Cuminum cyminum. L is one of the member Apiaceae family. This plant has anticancer and antioxidant traits. The main purpose of this research is cloning of flavone synthase I gene from Caraway Cumin (Cuminum cyminum) indigenous in Iran. In order to cloning FNSI after extraction of total RNA, cDNA Library were constructed using oligo dT primers and gene amplification of FNSI was performed. After cutting the amplified gene cloned in expression plasmid PET28a and was transferred to the DH5 bacterial utilizing by heat shock method. Screening of transformed bacteria was done on medium containing kanamycin and at least to confirm the gene cloning method for determination of the cloned genes, gene amplified of the gene using specific primers and cutting plasmid by specific restriction enzymes and analyzed of the products on agarose gel was used. In this experiment, after all the cloning steps, the 6kb and 1 kb bands were observed on the electrophoreses gel plasmid and the flavone synthase I gene, respectively, confirming the cloning of this gene in pET28a plasmid. Given that flavones are important in plants as well as being of high pharmaceutical value, having a cloned set of flavone synthase genes can be of high pharmaceutical and commercial value.
Keywords: Cuminum cyminum, Flavone synthase I, Cloning, Secondary metabolites
Edition: Volume 8 Issue 12, December 2019
Pages: 1924 - 1929
Make Sure to Disable the Pop-Up Blocker of Web Browser
Similar Articles
Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1
Research Paper, Biotechnology, India, Volume 13 Issue 7, July 2024
Pages: 1632 - 1634Antifungal Properties of Streptomyces sp. against Aflatoxin Producing Aspergillus Species
Medha Jyoti, Dr. Archana Kumari
Downloads: 2 | Weekly Hits: ⮙2 | Monthly Hits: ⮙2
Doctoral Thesis, Biotechnology, India, Volume 14 Issue 1, January 2025
Pages: 372 - 374Impact of Temperature and pH on Anti-Fungal Secondary Metabolites Production Ability of Streptomyces sp.
Medha Jyoti, Dr. Archana Kumari
Downloads: 4 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1
Review Papers, Biotechnology, India, Volume 10 Issue 11, November 2021
Pages: 619 - 621Review on Bryophyllum Pinnatum
Abhishek C. Gavali, Sarika T. Loni, Unnati S. Tipe
Downloads: 71 | Weekly Hits: ⮙2 | Monthly Hits: ⮙2
Informative Article, Biotechnology, France, Volume 7 Issue 8, August 2018
Pages: 578 - 581Biotechnology
Jean Marius D'Alexandris
Downloads: 146
Research Paper, Biotechnology, India, Volume 7 Issue 8, August 2018
Pages: 394 - 402Characterization and Molecular Analysis of NBS-LRR Class Resistance Gene Homologues Isolated from Zingiber spp. of North East India
Nongmaithem Chanu Lenbi, Huidrom Sunitibala Devi, Pratap Jyoti Handique