International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 173 | Views: 352

Research Paper | Medical Microbiology | Saudi Arabia | Volume 8 Issue 6, June 2019 | Popularity: 6.7 / 10


     

Phenotypical Detection of Extended Spectrum Beta-Lactamases Producing Escherichia coli, from Clinical Isolates in Riyadh Region, Al-Kharj City, Kingdom of Saudi Arabia

Eltayib Siddig Mohammed Saad, Adam Dawoud Abakar, Bakri Yousif Mohammed Nour, Hisham N. Altayeb, Makah Abdulmanan Badwi Ali


Abstract: Background and objective: the aim of the present study was to identify the phenotypical detection of extended spectrum beta-lactamases, (ESBL) Phenomenon among Escherichia coli isolated from clinical samples in King Khalid Hospital and Price Sultan Center, Alkharj City, Riyadh Region, Kingdom of Saudi Arabia. Materials and Method: A total of 174 isolates of Escherichia coli were isolated by standard microbiological method, and identified by BD Phoenix system ( Becton Dickinson, USA), full automated microbiological machine confirmed by API 20 E ( BioMrieux, Marcy LEtoile, France). the antimicrobial susceptibility pattern of the isolates was determined by BD Phoenix system confirmed by E. test ( Epsilometer test), (Oxioid, UK) according to CLSI guideline (2008). The Extended spectrum beta- lactamases (ESBL) producing Escherichia coli were screened by BD Phoenix system confirmed by double disk diffusion method. p- value < 0.05 was counted as statistically significant. Result: more than forty two percent was found to be positive for ESBL producers and 57.5 % as non-ESBL producers, urine samples were the most frequent in this study, males were more prevalent to ESBL producers than females, high resistant of ESBL producers was observed among different family of antibiotics including, pipracillin (76.3 %), ampicillin (76.3 %), tetracycline (62.7 %), ceftriaxone (60.0 %), cefuroxime (55.0 %), trimethoprime / sulphamexazole (54.8 %), azitronam (54.1 %) and amoxicillin / Clavulanic acid (53.6 %). Meropenes, imipenem, amikacin and tigycyline were the most active against ESBL producers. Conclusion: The prevalence of ESBL producers in this study was high compared with others studies performed in Saudi Arabia, so it requires sound infection control measures, antimicrobial management and measures to detect and control their spread should be considered.


Keywords: Escherichia coli, Antibiotics resistance, ESBLs detection, Clinical isolates


Edition: Volume 8 Issue 6, June 2019


Pages: 228 - 233



Make Sure to Disable the Pop-Up Blocker of Web Browser




Text copied to Clipboard!
Eltayib Siddig Mohammed Saad, Adam Dawoud Abakar, Bakri Yousif Mohammed Nour, Hisham N. Altayeb, Makah Abdulmanan Badwi Ali, "Phenotypical Detection of Extended Spectrum Beta-Lactamases Producing Escherichia coli, from Clinical Isolates in Riyadh Region, Al-Kharj City, Kingdom of Saudi Arabia", International Journal of Science and Research (IJSR), Volume 8 Issue 6, June 2019, pp. 228-233, https://www.ijsr.net/getabstract.php?paperid=ART20198426, DOI: https://www.doi.org/10.21275/ART20198426



Similar Articles

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Analysis Study Research Paper, Medical Microbiology, India, Volume 13 Issue 2, February 2024

Pages: 731 - 735

Urinary Tract Infections: Epidemiology, Sex Incidence & Correlation with Pyuria at a Tertiary Care Laboratory in New Delhi

Dr. Jayant Balani, Dr. Asha Bhatnagar, Puja Sharma

Share this Article

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Analysis Study Research Paper, Medical Microbiology, India, Volume 13 Issue 6, June 2024

Pages: 680 - 683

Microbial Profile of Corneal Scrapping Samples Received in Department of Microbiology in a Tertiary Care Hospital, Himachal Pradesh

Dr. Manvi Sharma, Dr. Dogra Kumari Deeksha, Dr. Rana Aditya, Dr. Sood Anuradha C, Dr. Kaur Isampreet

Share this Article

Downloads: 2 | Weekly Hits: ⮙2 | Monthly Hits: ⮙2

Comparative Studies, Medical Microbiology, India, Volume 11 Issue 6, June 2022

Pages: 1734 - 1738

Evaluation of in vitro Susceptibility of Multi Drug Resistant Uropathogenic Escherichia Coli (UPEC) to Fosfomycin: A Comparision of Determination Methods

Dr. Bijitatma Sahoo, Dr. Smitashree Chhualsingh, Dr. Susmita Kumari Sahu, Dr. Pradipta Kishore Sahoo

Share this Article

Downloads: 2 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Analysis Study Research Paper, Medical Microbiology, India, Volume 12 Issue 12, December 2023

Pages: 1664 - 1667

Changing Pattern of Antibiotic Profile of Gram Negative Blood Stream Infections in NICU in a Tertiary Care Hospital

Dr. Gurpreet Kaur, Dr. Kamaldeep Singh, Dr. Loveena Oberoi, Dr. Tavishi Oberoi

Share this Article

Downloads: 2 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 13 Issue 2, February 2024

Pages: 312 - 320

Study of Antibiotic Sensitivity and Resistance Pattern of Bacterial Isolates in Intensive Care Unit Setup of a Tertiary Care Hospital

Dr. Milan Nimbalkar

Share this Article
Top