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Research Paper | Biological Sciences | India | Volume 8 Issue 3, March 2019
Variation of Wild Cymbopogon martinii (Roxb.) in Different Ecotypes using Molecular Markers
Vijayalakshmi T. N. | Prof. Thara Saraswathi K. J.
Abstract: The study of the genetic diversity of wild plant populations is essential for their management and conservation. In-order to provide a better comprehension of the results obtained and a comparison between markers which will help to choose tools for future studies in natural populations of Cymbopogons. This study used both Isozymes and Amplified fragment length polymorphism (AFLP) to assess genetic diversity and genetic structure of six populations, pointing to similarities and divergence of each marker and evaluating the relative importance of the results for studies of population genetics and conservation. Suitable identification and characterization of medicinal plants/aromatic plants are necessary for conservation of plant resources, investigations of genes associated with desirable traits, and understanding of evolutionary relationships. Therefore, various molecular marker techniques such as AFLP and Isoenzymes have been improved to provide detail information about genomes. This brief review represents usage of these markers for molecular diversity analysis of medicinally important plants (SevgiMarakli 2018). Several DNA based marker systems are available for genetic finger printing of plants but information ontheir relative usefulness for characterization of Cymbopogon germplasm is lacking. The efficiency of AFLP markers for the assessement of genetic relationships. This species of different ecotypes, were investigated. Dendrogram is produced based on band pattern scores using UPGMA metho (Nirupama Lal and S. K. Awasti 2014). Genetic diversity of 6 accessions estimated using amplified Fragment length polymorphism (AFLP) marker technology and specific activity of Isoenzymes using spectrophotometric methods. Six ecotypes Cymbopogon martinii in the studyfive AFLP primer combinations generated the bands and showing average polymorphic. The UPGMA method was employed to construct a dendrogram based on the Jaccards similarity co-efficient. Based on these co-efficient it was concluded that these accessions show genetic variation. These results have significant implications in the crop breedingprogrammes, the results demonstrates the great value of AFLP and Isoenzyme markers in the management of genebanks. Several isoenzymes are present in plants and their relative composition changes during to exposure to stress. Several methods are available for the quantitative determination of the enzymes. Based on the spectrophotometric detection and quantification, the enzyme activity is calculated by determining the concentration compounds in solution (M. S. Negi, V. Sabharwalet al. , 2003)
Keywords: Wild Cymbopogon martinii, AFLP, Isoenzymes, Genetic diversity
Edition: Volume 8 Issue 3, March 2019,
Pages: 673 - 679