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Research Paper | Pharmaceutical Science | India | Volume 7 Issue 9, September 2018

Analytical Method Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Paracetamol and Tolperisone Hydrochloride Drug in Pure and Pharmaceutical Dosage Form

Yogesh Waghmare | Sonali Mahaparale

Abstract: Two methods for simultaneous estimation of Paracetamol and Tolperisone Hydrochloride in combined tablet dosage form have been developed using Buffer, Acetonitrile and Methanol as a solvent. The first UV spectrophotometric method was a determination using the simultaneous equation method at 246.0 nm and 260.0 nm. The second UV spectrophotometric method is the Q analysis (absorption ratio) method, which involves the formation of absorbance equation (isoabsorptive point) at 258.0 nm the maximum absorption of Tolperisone Hydrochloride. The linearity ranges for Paracetamol and Tolperisone Hydrochloride range of 26.96 80.88 g/ml and 12.40 37.21 g/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 102.03 3.86 and 98.92 0.89 for simultaneous equation method and 100.6 1.80 and 99.41 1.29 for Q analysis (absorption ratio) method for Paracetamol and Tolperisone Hydrochloride respectively. These methods are simple, accurate and rapid, those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.

Keywords: Paracetamol, Tolperisone Hydrochloride, Qanalysis spectrophotometric method ICH

Edition: Volume 7 Issue 9, September 2018,

Pages: 1341 - 1346