Polymerase Chain Reaction (PCR) based Diagnosis of Extra-Pulmonary TB in Clinical Samples by Targeting IS6110 Sequence

Suthar Jigar, Suthar Kanagee

Abstract: India has the highest burden of TB. Mycobacterium tuberculosis (TB) is one of the oldest and biggest killer of the humans compare to all other infections. Tuberculosis (TBC) is an infectious disease caused by some species of genus “mycobacterium”. Accurate and early detection of mycobacterium tuberculosis decrease incidence of tb. There is a several techniques available but PCR based technique is most common for diagnosis. TB is divided mainly in two parts that are Pulmonary (in side of lungs) and Extra-pulmonary (outside of lungs). We have focused on extra-pulmonary TB. A small amount of mycobacteria inhalation can able to infect us. Mycobacterium tuberculosis is a slow growing bacterium and doubles at every 18 to 24 hours. This research was based on PCR method. Different samples of extra-pulmonary were collected from the laboratories, which were taken for the diagnostic purpose. Those samples were checked for the absence or presence of the any mycobacterium species by amplifying the IS6110 sequence.

Keywords: TB, MTB, TBC, Mycobacterium tuberculosis, PCR