Antibacterial Potency of Sarang Semut (MyrmecodiaPendans Merr. & Perry) to Prevent Adhesion and Growth of Enterococcus faecalis

Cut Soraya, Hendra Dian Adhyta Dharsono, Dudi Aripin, Mieke H. Satari, Dikdik Kurnia, Danny Hilmanto

Abstract: Introduction. Enterococcus faecalis is one of the anaerobic facultative bacteria that causes root canal infections. The growth intensity and adhesion strength of E. faecalis against the host cell makes it difficult to eliminate. Previous studies have reported that extract of Sarang Semut (Myrmecodia pendansMerr. & Perry) has a promising sensitivity to E. faecalis, especially at high concentrations. Objective. The purpose of this study was to analyze the potential of extracts of Sarang Semut in preventing adhesion and growth of E. faecalis based on the time of incubation. Method. E. faecalis ATCC 29212. E. faecalis is equalized with Mc. Farlan 0, 5 (-1x108). Anti-adhesion activity of extract of Sarang Semut on E. faecalis ATCC 29212 was analyzed by spectrophotometry at wavelength 590 nm and the growth inhibitory activity was analyzed by using culture approach. Results were analyzed by One Way Anova test (p<0, 05). Result. Based on the incubation time (3, 6, 9, 12, 16, and 24 hours), the activity of the extract of Sarang Semut significantly showed the ability of E. faecalis to prevent adhesion. This result also correlated with decreased growth of E. faecalis colonies, particularly in the concentration of 100 % (g/ml) as the highest concentration. Conclusion. Based on the incubation time, the extract of Sarang Semut has ability to prevent both adhesion and growth of E. faecalis. This ability is shown primarily on the highest concentration of Sarang Semut extracts

Keywords: Growth, adhesion, Sarang Semut Extract, E faecalis