International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064

Downloads: 117 | Views: 157

Research Paper | Biotechnology | India | Volume 6 Issue 5, May 2017

Study of in-vitro Micro-propagation of Medicinally Important Plant Andrographis paniculata from its Different Parts

Singh Abhilasha | Awasthi Dr. Arpita

Abstract: Andrographis paniculata is a plant of high medicinal importance. Explants from leaves, stem internodes and seeds had shown different patterns of callus stimulation under similar conditions of light duration, temperature and humidity with fixed concentrations of IAA and kinetin in MS media. During micro propagation, most efficient callus formation was observed in explants prepared from seeds and internodes. Under influence of IAA and kinetin, most viable callus culture was observed in seeds and internodes of stem.18 of 25 nodal segments have developed callus of 4-6 mm with an efficiency of 72 %.21 of 25 seedlings have developed callus of 2-3mm with an efficiency of 84 %. During callus development, few seedlings also exhibited some shoot development.11 of 25 explants prepared from leaves have developed callus of 3-5mm with an efficiency of 44 %.

Keywords: Andrographis paniculata, MS Medium, micro-propagation, in-vitro, callus

Edition: Volume 6 Issue 5, May 2017,

Pages: 1060 - 1063

How to Download this Article?

Type Your Email Address below to Receive the Article PDF Link

Verification Code will appear in 2 Seconds ... Wait