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Research Paper | Zoology | India | Volume 6 Issue 3, March 2017
Analysis of Microbes and their Enzyme in Achatina fulica
Abstract: Molluscs forms one of the imperative biotic components in an ecosystem playing vital roles in the ecosystems and are often used as bioindicators of the ecosystem health. As bioindicators, they uptake environmental toxins through food chain from their surroundings. The heavy metals such as zinc, cadmium, lead and copper can be taken up by land snails, as shown by laboratory experiments wherein they were fed toxin laced lettuces. In addition, molluscs are useful indicators for biological assessment of water quality monitoring. But in more recent lab. tests, snails incorporate showed that cadmium from their surroundings when they are living in contaminated soil. Achatina fulica snails are polyphagous and along with organic detritus, preferred plant species, ranging from coffee and bananas in the tropics, potatoes and tobacco in more temperate zones. The snails are voracious eaters and competitors of native snails as well as habitat modifiers. Their importance in agricultural systems, gardens and as agents for the reduction of biological diversity is increasing at an alarming rate making the mas one of the most important pests worldwide. There are limits to the duration of aestivation that can be tolerated by land snails and mortality eventually increases as aestivation is prolonged. While physiological alterations that occur during aestivation have received much interest. Lignocellulosic biomass is degraded with the cooperation of many microorganisms, producing a variety of cellulolytic and hemi-cellulolytic enzymes under aerobic and anaerobic conditions. Glycoside hydrolases from bacteria are assembled in multienzyme complexes that provide increased synergy, stability and catalytic efficiency. Multifunctional, harbouring both endoglucanase and xylanase activities in the polypeptide isolated and identified the cellulolytic bacteria from the gastrointestinal tract of Achatina fulica and analyse strains confirmed using bioinformatics tools. Cellulose digesting enzyme production of cellulolytic bacteria isolated from the gastrointestinal tract of Achatina fulica were confirmed by zymogram and cladogram analysis.
Keywords: Xylanase, cellulolytic bacteria, Achatina fulica, FPase, CMCase, zymogram, cladogram
Edition: Volume 6 Issue 3, March 2017,
Pages: 1897 - 1903