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Research Paper | Biochemistry Science | India | Volume 6 Issue 1, January 2017

HPLC, UV Spectrum Analysis of Polyherbal Formulation and DNA Damage in Hepatoprotective CCl4 Induced Albino Rats

A Dhinek | C Sowmia

Abstract: The objective of this study was to investigate the hepatoprotective activity of ethonolic extract of polyherbalformulation (Solanumxanthocarpum, Glycyrrhizaglabra, Ricinuscommunis, and Phyllanthusniruri leaves) against carbon tetrachloride (CCl4) induced hepatotoxicity. The plants were dried in shade then powdered, extracted with ethanol. Preliminary phytochemical tests were done with the ethanolic extract showed presence of mostly all the compounds. The hepatoprotective activity of the ethonolic extract was assessed in CCl4 induced hepatotoxic albino rats. HPLC and UV spectrum analysis revealed to identify and to quantify each component. The present study that exhibit free radical scavenging properties. DNA damage in liver was also evaluated using Comet assay. The DNA damages and the length are tested, Progression of liver damage induced by CCl4 in albino rats can be intervened using the polyherbal extract and these effects are comparable to those of silymarin, polyherbal treatments effectively restored these measurements closer to their normal levels.

Keywords: Hepatoprotection, Hepatotoxicity, CCl4, Sylimerin, Polyherbal drugs

Edition: Volume 6 Issue 1, January 2017,

Pages: 129 - 132