The Anti-angiogenic Activity of Matricaria Chamomilla Flowers Extraction

Adeeb A. AL-zubaidy, Hayder B Sahib, Safaa H. Ganduh

Abstract: Background The Matricaria chamomilla is widely used in Asian countries as the traditional medicine to treat various diseases. Objective The possible anti-angiogenic activity of Matricaria chamomilla flowers extracts was aimed to be investigated. Subjects and methods The powder of the Matricaria chamomilla flowers was extracted sequentially with petroleum ether, chloroform, methanol and water using the cold method maceration as extraction process. The ex vivo rat aorta ring assay was used to screen the extracts for possible antiangiogenesis activity, this assay was also used to determine the doseresponse effect of the active extract by preparing serial concentrations. Free radical scavenging activities of the extracts were determined using 1, 1diphenyl2picrylhydrazyl) assay (DPPH) Results The obtained data revealed that the four extracts exhibited significant inhibition of blood vessels growth when they were compared to the negative control (received DMSO 1 %) (P<0.05) amidst them methanol extract was shown to have significant inhibition of blood vessels growth with comparable effect to positive control (acetylsalicylic acid). Methanol extract of Matricaria chamomilla flowers exhibited a significant dosedependent antiangiogenesis effect with IC50 (29.85g/ml). Furthermore, methanol extracts exhibited a significant free radical scavenging activity (P<0.05) with IC50 (81 g) compared to other extracts. Conclusion the results revealed that methanol extracts of Matricaria chamomilla flowers exhibited the most significant antiangiogenesis activity and this activity may be due to the high free radical scavenging capacity

Keywords: Anti-angiogenesis, Matricaria chamomilla, free radical scavenging activity