Perigrinations of Real Time PCR

Mohammed Khwaja Moinuddin, Dola Binoy, L Karthik Prasad

Abstract: Polymerase chain reaction (PCR) is a diversifying molecular biology techniques in nucleic acid analysis. Real time PCR is the peregrinating form of polymerase chain reaction with accurate quantitation of the cells of individual bacterial species in given dental samples providing the bacterial etiology of endodontic and periodontic infections. Real-time PCR offers efficient, sensitive and reliable approach to quantitation. In addition to snap shot of state of art of real time PCR, here we review the principles of real time PCR, methods of quantitative detectionand applications of Real-Time PCR in dentistry.

Keywords: Polymerase chain reaction-PCR, real time PCR, Reverse transcription PCR RT-PCR, Endodontic infection, bacterial identification, double-stranded DNA dsDNA, Fluoroprobes, Amplicons, Quencher