Screening and Optimization of L? Asparginase Produced by Bacteria Isolated from Soil of Solapur Region

Afshaan Naaz Shaikh Khaleed, R. M. Khobragade

Abstract: L-asparaginase (E. C No 3.5.1.1) is a potential anti-carcinogenic enzyme. Seven different soil samples from Solapur regions were screened for potential producers of L-asparaginase using modified Czapek doxs agar containing L-asparagine and phenol red as indicator with pH 7. The strain isolated from slaughter house soil sample (S-2) showed the maximum enzyme activity. The enzyme production was carried out by submerged fermentation. The maximum enzyme activity was 103.7 U/ml was showed by 24 hrs old culture at 37o C.

Keywords: L-asparaginase, anticancer, bacteria