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M.Tech / M.E / PhD Thesis | Medicine Science | Iraq | Volume 5 Issue 9, September 2016
Antiangiogenic Activity of Iraqi Anabasis articulata Stems In vivo Study
Waleed K. Abdulsahib [3] | Abdulkareem H. Abd | Ban Jumaa Qasim
Abstract: background Anabasis articulata, also called Eshnan, Ajrem or Berry bearing glasswort, is widely distributed in Syrian, Algerian, Egyptian and Iraqi desert. Anabasis articulata is broadly used in folk medicine to treat diabetes, fever, eczema and kidney infections. Objective to examine the antiangiogenic action of Anabasis articulata in vivo. Subjects and methods The powder of the Anabasis articulata stems was sequentially extracted with four solvent types of different polarity. Eggs were gestated for three days, small whole prepared on the fine pinpoint. The following day, the eggs sac was penetrated and a small frame was made in the shell. Eggs were returned to the incubator until day 10 of chick embryo growth, methanol extract was moved to the Chick Embryo Chorioallantoic Membrane (CAM), and eggs incubated for 72 hours (n = 6), the zone of inhibition was estimated as mean of inhibition area. Functional groups of the chemicals component private to the extract has been recognized by Fourier transform infrared spectroscopy FT-IR and Gas chromatography mass spectrometry was utilized to recognize the most likely relevant agent. Results The results displayed that the zones of inhibition area range between 7 and 10 mm. FT-IR displayed that some of the recognized functional group may relate to flavones, coumarins, alkalines, saponins and tannins. Scopoletin, 2-Methoxy-4-vinylphenol, glycine and 1, 2-dimethyl- Piperidine, have been recognized in methanol extract. Conclusion the results revealed that the mechanism of anti-angiogenic activity for the methanol extract of the Anabasis articulata stems may be related to these substances that have the capacity to block the VEGF- receptor, thereby inhibiting the angiogenesis.
Keywords: Anabasis articulata, in vivo study, anti-angiogenesis, CAM assay, Scopoletin, 2-Methoxy-4-vinylphenol, glycine and Piperidine
Edition: Volume 5 Issue 9, September 2016,
Pages: 220 - 226