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Research Paper | Pharmacy | Senegal | Volume 12 Issue 10, October 2023
Phytochemical Screening, Secondary Metabolites Assay, Antioxidant, Antibacterial and Antifungal Activity of Clove Buds (Syzygiumaromaticum) Extracts
Ousmane Niass [2] | Issa Samb | Betemondji Desire Diatta | Abdoulaye Diop | Assane Dieng | Khadim Diongue | Amadou Diop [2] | Serigne Omar Sarr [2] | Bara Ndiaye [2] | Yerim Mbagnick Diop [2]
Abstract: Clove buds (Syzygiumaromaticum) are one of the most widespread plants and their essential oil is by far the most widely used part. In this study, which first focused on the phytochemical characterization of aqueous, methanolic, and acetone extracts, the methanolic one is the richest and contains all the secondary metabolites sought, with the exception of leucoanthocyanins and emodins. Then, an assay of alkaloids, flavonoids, and polyphenols is done by colorimetric methods. The results showed that aqueous infused is richer in alkaloids, followed by methanolic extract with concentrations of 6.71 ?g/mg and 2.55 ?g/mg respectively. For the flavonoid composition, organic solvent extracts are the richest, namely acetone extract with a concentration of 132.66 ?gQE/mg, followed by methanolic extract at 140.84 ?gQE/mg and the quantification of polyphenols has also shown that methanolic extract is richer, followed by infused extract with concentrations of 438 and 414.5 ?gAGE/mg. In addition, the study of antioxidant activity by the ABTS method showed that infused is the most active extract with an IC50 of 1.6 ?g/ml lower than that of ascorbic acid used as standard. And finally, for the study of antimicrobial activity, extracts are more active against Staphylococcus aureus, against which MICs of 5.7 ?g/ml are obtained with the aqueous fraction of the infused and 11.5 ?g/ml with the ethyl acetate fraction. Against Candida albican the smallest MIC is obtained with the ethyl acetate fraction which is equal to that of Neomycin B sulfate and is 187 ?g/ml.
Keywords: Clove dubs, alkaloids, polyphenols, antibacterial, antifungal, antioxidant
Edition: Volume 12 Issue 10, October 2023,
Pages: 1900 - 1905