Shraddha Hatangadi, Shashank More, Bhagyashree Ghag, Nirav Bhatia, Madhavi Indap
Abstract: Natural products (NP) have been always preferred over the synthetic products due to various side effects it brings along. Natural products have been a source of new drugs since few decades now. The current focus of NP research has been shifted towards marine ecosystem. Secondary metabolites of marine organisms have been found to possess antibiotic, antiviral and anti-cancer activity. Lingula anatina commonly known as lamp shell has been found to tolerate high salinities, which might be an indication of presence of some potential bioactive compounds. Hence, our present study is based on Cytotoxic and genotoxic potential. Chromosomal aberration and mitotic index test were applied to evaluate genotoxic potential of the extract using Allium cepa. root model. Cell viability was assessed on tumor and non-tumor cell lines such as MCF-7, A549 and CHO by MTT assay. Our study revealed that mitotic index decreases with increase in concentration from 100µg/ml to 400µg/ml. Maximum breaks were observed at 400µg/ml conc. Spindle abnormalities along with C-Mitosis, Vagrant and Lagging Chromosome, Stickiness, breaks and disoriented, fragmented chromosomes were also observed. Extract exhibited cytotoxicity against Breast Carcinoma cell line (MCF-7) and the IC-50 value was 605.60µg/ml. The Methanolic extract of Lingula anatina possesses cytotoxic and genotoxic potential which could be considered as a future drug in medicine.
Keywords: Natural Products, Lingula, Allium cepa, Cytotoxicity, MTT Genotoxicity, Aberrations