Reena Bahan, Shashidhar Singh
Abstract: This paper is concerned with the estimation of proteins in the body fluids of insect. For estimation of proteins in the body fluids of Coccinella septmpunctata, commonly called ?Ladybird beetle? Biuret method is used. The weighed insects were narcotized and macerated in 3 ml of double distilled water. The macerate was centrifuged to separate serum extract. The protein content of the serum extract was estimated by colorimeter. Total serum protein content was calculated by the following formula: Serum Protein,gm/dl= (OD Test)/(OD Std.) X6 . O.D. Test = depth of the colour formed by the testing fluid. O.D. Std. = depth of the colour of the standard fluid. The average total serum protein content in male insects of C. septempunctata was 5.99 gm/dl and that in female insects was 6.8 gm/dl which is slightly greater than that in males.
Keywords: Estimation, serum extract, protein content, male, female, Coccinella septempunctata, Biuret method, centrifuge, colorimeter