Abstract: Modified NaOH method is the easiest and cheapest genomic DNA isolation from Okra leaf samples for PCR amplification. DNA isolation from Okra is quite difficult job due to polysaccharides and polyphenols. Even isolated DNA is not compatible with thermal reaction. A standard protocol of Wang et al. (1993) was reviewed and modified for DNA extraction from Okra leaf tissues within a short time in an effective way which is quite cheap. This method is also effective for molecular characterization of Okra and could be used for molecular characterization in other mucilaginous crops.
Keywords: genomic DNA, okra, cost-effective, easy, polysaccharides, compatible for PCR