Molecular Characterization and Cytotoxic Activity on HCT-15 and HepG2 of L-asparaginase Producing Bacteria from Mugil cephalus

Sharmila Rajan | B. Deivasigamani

Abstract: Over the recent years the active role of enzymes in every field of work has tremendously increased. Medicinal field is one such area which harbours the enzyme for the treatment of many diseases. L-asparaginase is a therapeutic enzyme which has the ability to treat acute lymphoblastic leukaemia and lymphosarcoma. It converts the free asparagine to aspartic acid and ammonia. Present paper showed the biochemical analysis and the molecular characterization of maximum enzyme producing bacterial strain LAS-8.16S rRNA of the strain was sequenced and identified as Enterobacter cloacae. The precipitated enzyme sample was further analysed for its cytotoxic activities.

Keywords: L-asparaginase, acute lymphoblastic leukaemia, Enterobacter cloacae, HCT 15 cell line, HepG2 cell line

Edition: Volume 7 Issue 9, September 2018,

Pages: 941 - 944