Downloads: 125
Research Paper | Botany | India | Volume 5 Issue 8, August 2016
In vitro Plant Regeneration from the Nodal Explants of Spermacoce articularis L. f.
Biula Preethi C. | Velayutham P. [2] | Karthi C. [3]
Abstract: An in vitro regeneration of plantlets from the nodal explants of Spermacoce articularis L. f. , a member of Rubiaceae, has been conducted. Nodal regions with axillary buds were cultured on MS medium supplemented with different concentrations of BAP and KIN ranging from 5 to 25 M alone or in combination of both. Among the two cytokinins tested 10 M BAP was found to be the best for shoot proliferation. However, combination of KIN with 10 M BAP further enhanced the rate of shoot proliferation. Maximum number of 31.8 shoots per node with 100 % shoot induction frequency were observed on MS medium containing 10 M BAP and 4 M KIN. The regenerated shoots were cultured on half strength MS medium containing different concentration of IAA, IBA and NAA for root induction. Among these three auxins, 4 M IBA was found to be the best hormone for rooting. The well rooted plantlets were successfully hardened with 80 % survival rate.
Keywords: Spermacoce articularis, Nodal Segments, Direct Regeneration, Cytokinins, Rooting, Auxins
Edition: Volume 5 Issue 8, August 2016,
Pages: 226 - 230
Similar Articles with Keyword 'Nodal Segments'
Downloads: 112
Research Paper, Botany, India, Volume 5 Issue 7, July 2016
Pages: 1986 - 1988High Frequency Regeneration in Important Medicinal Plant Uraria PICTA JACQ DC
Avinash Jadhav [2] | Rohit Shete [2] | Narayan Pandhure [10]
Downloads: 135
Research Paper, Botany, India, Volume 4 Issue 3, March 2015
Pages: 63 - 67In vitro Shoot Multiplication of Medicinally Important Caralluma stalagmifera Fischer
V. Raja Sreelatha | M. Thippeswamy | T. Pullaiah [3]