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Research Paper | Microbiology | Egypt | Volume 6 Issue 1, January 2017

Efficacy of Antibiotics Combined with Biosynthesized Silver Nanoparticles on some Pathogenic Bacteria

Ekbal H. Abdel Hafez | Entesar A. Ahmed | Heba S. Abbas | Rawheya A. Salah El Din

Abstract: Biosynthesis of Silver Nanoparticles (AgNPs) has received great attention due to their numerous advantages like being rapid, simple and eco-friendly protocol. In the present study, we used cultures supernatant of pathogenic bacteria and fungi like Staphylococcus aureus 29737 ATCC, Staphylococcus epidermidis 12228 ATCC and Klebsiella pneumoniae 700603 ATCC, as well as cell free filtrate of Fusarium sp. and Aspergillus flavus for rapid synthesis of silver Nanoparticles. UV- visible spectrometry study revealed the plasma resonance from 420 to 440 nm. Transmission electron microscope of AgNPs showed the mean average size of most particles was 11, 29.9, 16.8, 23.4 and 23.4 nm for Staphylococcus aureus, Staphylococcus epidermidis and Klebsiella pneumoniae, Fusarium sp. and A. flavus respectively. The Fourier Transmittance Infrared spectrum (FT-IR) confirmed the reduction of the silver ions and forming stable and control shaped silver nanoparticles due to the protein component resulting from the enzyme nitrate reductase. Results of this study revealed that the synthesized silver nanoparticles have potential antibacterial activity and were also capable of enhancing the efficiencies of some commercial antibiotics.

Keywords: Biosynthesis of silver nanoparticles, UV-Vis spectrophotometer, FTIR, TEM antibacterial, antibiotic enhancer

Edition: Volume 6 Issue 1, January 2017,

Pages: 1294 - 1303

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