Normal Saline Rehydration Technique or Carnoy's Solution - What to Choose for Hemorrhagic Fluid Cytology

Mudit Sharma ^[2] | Rateesh Sareen ^[4] | Ranu Sharma | Anjali Sharma ^[5]

Abstract: It is not uncommon to receive hemorrhagic fluids for cytological examination. The red blood cells impair diagnostic efficacy of smears. The body fluid can be processed in many ways- NSRT (Normal saline rehydration technique) is one such technique. The present study was done to evaluate quality of smears prepared by NSRT and Carnoy�s fixation. The smears were stained by Hematoxylin & Eosin and Leishman stain. The smears were analyzed for nuclear, cytoplasmic, background and quantity of cells. Scores were given from 0 to2. A total of 30 hemorrhagic fluids were taken. NSSRT showed better cell lysis and better background in comparison to Carnoy�s fixative on Hematoxylin & Eosin stain and also on Leishman stain. Hematoxylin & Eosin was better than Carnoy�s fixative technique. NSRT is better technique as it is simple, cost-effective and faster than Carnoy�s technique for hemorrhagic fluids.

Keywords: NSRT, Carnoys fixative, Hemorrhagic body fluid

Edition: Volume 6 Issue 5, May 2017,

Pages: 1416 - 1419