Comparison of NS1 Antigen and Antibody Detection Method in Early Diagnosis of Dengue Infection

Neeti Mishra, Shailendra Mohan Tripathi, Vineeta Khare, Sana Islahi, Mohd Gulrez, Priyanka Shukla, Shadma Yaqoob, Y. I. Singh

Abstract: Background and Aim The present study was carried out to evaluate whether NS-1 antigen help in early diagnosis of dengue infection as compared to Anti IgG/IgM antibody. Methodology For this purpose a total of 170 clinically suspect patients were enrolled in the study. All the patients were subjected to NS-1 Antigen and anti-IgG/IgM assessment using SD-Bioline Dengue Duo combination kit. Data was analyzed using SPSS version 15.0. Chi-square test, 't'-test, level of agreement (?) and sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the results was calculated. Results Positivity rate was 53.5%. Maximum positivity was observed for NS-1 (n=74, 43.5%) followed by IgM (n=71, 41.8%). NS-1 antigen had a higher sensitivity rate for early detection (

Keywords: Dengue, Dengue infection, Early diagnosis, NS-1 Antigen, anti IgG/IgM