Estimation of Antioxidants (Flavonoids) in Ailanthus excelsa and Balanites aegyptiaca and Effect of Growth Regulators and Salts on Antioxidants in vitro

Kavita Sharma, Asha Goswami

Abstract: Ailanthus excelsa and Balanites aegyptiaca are two wild growing common plant species of desert, having medicinal importance. Unorganized tissues of these useful plants were established on MS medium supplemented with 1.0mg/L BAP+1.5mg/L 2, 4-D and 1.5mg/L BAP +2.0mg/L 2, 4-D respectively (standardized MS medium). Parts of established tissues were transferred to standardized (Sd) MS medium fed with various concentrations (1, 2, 3 mg/L) of growth regulators ( IAA, NAA) and (10, 20, 30mg/L) salts ( NaCl, KCl) separately. Tissues at the maximum GI ( in all samples) were harvested, dried, powdered and analyzed for estimation of flavonoids. Maximum amount of flavonoids was calculated in callus fed with 1mg/L IAA, NAA and 10 mg/L NaCl and KCl in A. excelsa and B. aegyptiaca.

Keywords: Ailanthus excelsa, Balanites aegyptiaca, antioxidants, growth regulators, salts