66 kDa Protein Isolated from 24,000 g Fraction of Plasmodium berghei Exhibits Humoral Immune Response

Nisha Devi, H. S. Banyal

Abstract: The various antigens involved in the induction of humoral immune response provide the opportunity for development of an effective malaria vaccine. In the present study, the immunogenicity and protective efficacy of 24, 000 g fraction was evaluated in rodent mouse model. A 66 kDa protein was eluted through immunoadsorption using 24, 000g fraction. The eluted protein was characterized by SDS-PAGE and mice immunized exhibited strong humoral immune response analysed by ELISA, IFA, DOT-ELISA and in vitro invasion inhibition assay. About fifty percent inhibition of parasite propagation was observed in vitro. This protein appears to be an important constituent of the parasite for antimalarial studies.

Keywords: Plasmodium berghei, malaria, 24, 000g fraction, 66 kDa protein