Identification, Isolation and Estimation of Flavonoids and Effect of Growth Regulators and Salts on Flavonoids in Aegle marmelos and Moringa oleifera in vitro

Kavita Soni, Asha Goswami

Abstract: Unorganized tissues of medicinally useful plants Aegle marmelos and Moringa oleifera were established on MS medium supplemented with 1mg/L BAP+2mg/L 2, 4-D and 1.5mg/L BAP +1.5mg/L 2, 4-D respectively (standardized MS medium). Parts of established tissues were transferred to standardized (Sd) MS medium fed with various concentrations (1, 3, 5 mg/L) of growth hormones ( IAA, NAA) and (10, 20, 30mg/L) salts ( NaCl, KCl) separately. Tissues at the maximum GI ( in all samples) were harvested, dried, powdered and analyzed for estimation of flavonoids. Maximum amount of flavonoids was calculated in callus fed with 2mg/L IAA, NAA and 10mg/L NaCl and KCl in A. marmelos as wll as M. oleifera.

Keywords: Aegle marmelos, Moringa oleifera, flavonoids, growth regulators, salts