International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064

Downloads: 114 | Views: 153

Research Paper | Chemistry | India | Volume 4 Issue 6, June 2015

Rp-Hplc Method Development and Validation for the Analysis of Pharmaceutical Drugs- 60 % Sodium Lactate

Dr. Bhaskarrav Makwana | Dr. V. G. Patel | Vipalben Patel | Ami Patel

Abstract: A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of 60 % SODIUM LACTATE NaC3H5O3. Isocratic elution at a flow rate of 1.2ml /min was employed on a symmetry phenomenex hypersil L17 (300mm x 7.80 mm, 8 m- rezex RHM - monosaccharide H+) column at ambient temperature. The mobile phase consisted of 0.1M Sulphuric acid. The UV detection wavelength was at 210 nm. Linearity was observed in concentration range of 0.001 - 0.005gm/ml. The retention time for 60 % Sodium Lactate was 4.0 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of 60 % Sodium Lactate.

Keywords: Sodium Lactate, HPLC Method, Development, 210nm

Edition: Volume 4 Issue 6, June 2015,

Pages: 1532 - 1535

How to Download this Article?

Type Your Email Address below to Receive the Article PDF Link

Verification Code will appear in 2 Seconds ... Wait