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Research Paper | Chemistry | Volume 4 Issue 6, June 2015 | Pages: 1532 - 1535 | India
Rp-Hplc Method Development and Validation for the Analysis of Pharmaceutical Drugs- 60 % Sodium Lactate
Abstract: A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of 60 % SODIUM LACTATE NaC3H5O3. Isocratic elution at a flow rate of 1.2ml /min was employed on a symmetry phenomenex hypersil L17 (300mm x 7.80 mm, 8 m- rezex RHM - monosaccharide H+) column at ambient temperature. The mobile phase consisted of 0.1M Sulphuric acid. The UV detection wavelength was at 210 nm. Linearity was observed in concentration range of 0.001 - 0.005gm/ml. The retention time for 60 % Sodium Lactate was 4.0 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of 60 % Sodium Lactate.
Keywords: Sodium Lactate, HPLC Method, Development, 210nm
How to Cite?: Dr. Bhaskarrav Makwana, Dr. V. G. Patel, Vipalben Patel, Ami Patel, "Rp-Hplc Method Development and Validation for the Analysis of Pharmaceutical Drugs- 60 % Sodium Lactate", Volume 4 Issue 6, June 2015, International Journal of Science and Research (IJSR), Pages: 1532-1535, https://www.ijsr.net/getabstract.php?paperid=SUB155525, DOI: https://dx.doi.org/10.21275/SUB155525