Immobilization of Little Millet (Panicum sumatrense) ?-amylase

B. Usha, K. P. J. Hemalatha

Abstract: Alpha amylase (1, 4--D-glucan glucanohydrolase, EC 3.2.1.1) from germinating little millet seeds purified to homogeneity was entrapped in 2 % calcium alginate beads and gave immobilization yield of 65 %. It was observed that entrapment of -amylase was not affected by pH. Temperature optima of immobilized enzyme was higher compared to free enzyme. The enzyme on immobilization showed an apparent Km of 2.810-3g/ml. Immobilized enzyme showed the highest operational stability for upto 7 reuses by retaining 72.5 % of residual activity.

Keywords: amylase, little millet, immobilized, calcium alginate, entrapment

How to Cite?: B. Usha, K. P. J. Hemalatha, "Immobilization of Little Millet (Panicum sumatrense) ?-amylase", Volume 4 Issue 6, June 2015, International Journal of Science and Research (IJSR), Pages: 150-154, https://www.ijsr.net/getabstract.php?paperid=SUB155061, DOI: https://dx.doi.org/10.21275/SUB155061