Analysis of Free Amino Acid in Different Male Morphs of Callosobruchus Analis

Deepak Kumar

Abstract: The Present studies deals with the analysis of free amino acids in the development stages and also the various morphs of adult males morphs I-V of Callosobruchus analis by paper chromatography technique. For identification of amino acids in each band, their Rf value and Colours were compared with those of stranded map of known 20 amino acids. The colour intensity of bands was also recorded. The five morphs I-V of adult males comprise 9, 11, 8, 8 and 8 spots respectively. The amino acid pattern in the various morphs of adult males of Callosobruchus. analis has revealed difference in them. The difference observed during the present studies has been discussed in relation to their role in various life processes like metamorphosis, sex differentiation and polymorphism.

Keywords: Chromatography, Rf value, callosobruchus analis, Morph