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Research Paper | Veterinary and Animal Science | Volume 15 Issue 5, May 2026 | Pages: 988 - 993 | India
Molecular and Pathological Characterization of Mycobacterium Avium Subspecies Paratuberculosis Infection in Buffaloes of Jabalpur Region
Abstract: Background: Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic and debilitating disease in ruminants called paratuberculosis or Johne?s disease. It not only affects production of animals but also downturns the economy of the country. Thus, the aim of the present study was detection and documentation of MAP infection in buffaloes of Jabalpur region of Madhya Pradesh, India. Methods: A total of 148 buffaloes were screened in the study and 55 faecal, 25 blood and 20 tissue samples were collected from suspected animals. Preliminary test was performed with Ziehl-Neelsen (ZN) staining of faecal and tissue samples followed by indirect ELISA, histopathology and IS900 PCR analysis of ZN positive samples. Result: The results of present study revealed 20% seropositivity in buffaloes of the region. Further, 28.57% ZN positive faecal samples and 100% ZN positive tissue samples were found positive in IS900 PCR. The most significant pathological findings included gross thickening, congestion and severe corrugation on mucosal surface of ileo-caecal region of intestine. Microscopically, granulomatous reactions with cellular infiltration were observed in various regions of the intestine. Therefore, a conclusion of moderately prevalent MAP infection with moderate to severe pathological changes in buffaloes of the Jabalpur region of Madhya Pradesh can be drawn.
Keywords: Paratuberculosis, Johne?s disease, buffalo health, MAP infection, PCR detection, pathological changes
How to Cite?: Yash Kumar Verma, Yamini Verma, Apeksha Khare, Amishi, Madhu Swamy, Anju Nayak, "Molecular and Pathological Characterization of Mycobacterium Avium Subspecies Paratuberculosis Infection in Buffaloes of Jabalpur Region", Volume 15 Issue 5, May 2026, International Journal of Science and Research (IJSR), Pages: 988-993, https://www.ijsr.net/getabstract.php?paperid=SR26514231046, DOI: https://dx.dx.doi.org/10.21275/SR26514231046