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Research Paper | Pharmacy | Volume 15 Issue 4, April 2026 | Pages: 1053 - 1058 | India
Analysis of Paracetamol, Diclofenac Sodium and Caffeine in Mixture by Validated First Derivative Spectrophotometric Method
Abstract: For the simultaneous measurement of paracetamol (Para), diclofenac sodium (Diclo), and caffeine (Caff) in their combination without previous separation, a straightforward, precise, and economical first-derivative UV spectrophotometric approach has devised and validated. The approach is based on the first-derivative spectra and unique UV absorption properties of the three medications. In order to reduce mutual interference, paracetamol was measured at 209 nm, diclofenac at 283 nm, and caffeine at 318 nm. The method's linearity, accuracy, precision, limit of detection, and limit of quantification were all verified in accordance with ICH recommendations. With correlation coefficients less than 0.99, the calibration curves were found to be linear in the concentration ranges of 10-60 microgram per milliliter for paracetamol, 4-24 microgram per milliliter for diclofenac, and 2-12 microgram per milliliter for caffeine. While precision experiments produced %RSD values less than two percent for both intra-day and inter-day measurements, recovery studies verified accuracy in the range of 98-102%. For regular quality control analysis of combination Para-Diclo-Caffeine formulations in pharmaceutical dosage forms, the established first-derivative approach is quick and does not need chromatographic separation.
Keywords: Paracetamol, Diclofenac Sodium, Caffeine, UV Spectrophotometry, First Order Derivative, Validation
How to Cite?: Afzal N. Saiyad, Satish A. Patel, "Analysis of Paracetamol, Diclofenac Sodium and Caffeine in Mixture by Validated First Derivative Spectrophotometric Method", Volume 15 Issue 4, April 2026, International Journal of Science and Research (IJSR), Pages: 1053-1058, https://www.ijsr.net/getabstract.php?paperid=SR26418213841, DOI: https://dx.doi.org/10.21275/SR26418213841