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Analysis Study Research Paper | Nanotechnology | Volume 15 Issue 2, February 2026 | Pages: 975 - 978 | India
Green Synthesis, Characterization, and Antimicrobial Evaluation of Silver Nanoparticles Using Azadirachta indica (Neem) Leaf Extract: A Sustainable Approach to Nanotechnology
Abstract: The escalating demand for environmentally benign nanomaterials has propelled the adoption of green synthesis methods for metallic nanoparticles. This study presents a facile, eco-friendly biosynthesis of silver nanoparticles (AgNPs) utilizing aqueous leaf extract of Azadirachta indica (Neem), a widely available medicinal plant in India. The synthesis was optimized by varying reaction parameters such as temperature, pH, reaction time, and extract concentration. The formation of AgNPs was confirmed by a characteristic surface plasmon resonance (SPR) peak at 420-440 nm in UV-Vis spectroscopy. The nanoparticles were spherical to oval, with an average size of 22?30 nm, as determined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Fourier-transform infrared (FTIR) spectroscopy revealed the involvement of phytochemicals like phenols, flavonoids, and proteins in reduction and stabilization. X-ray diffraction (XRD) confirmed the face-centered cubic crystalline structure. The synthesized AgNPs exhibited potent antimicrobial activity against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria, with inhibition zones ranging from 15?25 mm. This research underscores the potential of plant-mediated AgNPs as sustainable alternatives for biomedical and agricultural applications, aligning with global sustainability goals.
Keywords: Green synthesis, Silver nanoparticles, Azadirachta indica, Anti-microbial activity, Characterization, Sustainable nanotechnology
How to Cite?: Dr. Moparthy John Paul, P. Sudhakar, "Green Synthesis, Characterization, and Antimicrobial Evaluation of Silver Nanoparticles Using Azadirachta indica (Neem) Leaf Extract: A Sustainable Approach to Nanotechnology", Volume 15 Issue 2, February 2026, International Journal of Science and Research (IJSR), Pages: 975-978, https://www.ijsr.net/getabstract.php?paperid=SR26216125247, DOI: https://dx.dx.doi.org/10.21275/SR26216125247