Evaluating the Efficacy of Serodiagnosis Technique of Dengue Virus Infection: An Epidemiological Study and Surveillance Analysis in Dimapur, Nagaland, India
Abstract: This study assessed the serodiagnostic and epidemiological characteristics of dengue virus (DENV) infection in Dimapur, Nagaland, India. A prospective study was conducted at the Department of Microbiology, District Hospital, Dimapur, from March 2023 to February 2024. Serum samples from 7,074 patients with suspected dengue infection were screened using rapid diagnostic tests for NS1 antigen and IgM antibodies, followed by NS1 and IgM ELISA confirmation. Selected samples were analyzed by RT-PCR for DENV serotyping. ELISA confirmed dengue infection in 2,010 cases, including 1,461 NS1-positive and 549 IgM-positive samples. Cases increased during the post-monsoon period, with the highest number recorded in October. Infection was more common among males and was most frequent in individuals aged 21-30 years. Among 39 samples suitable for molecular analysis, DENV-2 was detected in 13 samples, while 10 demonstrated co-infection with DENV-1 and DENV-2. Environmental assessment identified water logging, swamps, and mosquito breeding sites as potential contributors to transmission. The findings indicate substantial dengue transmission in Dimapur, characterized by seasonal and demographic variation and circulation of multiple DENV serotypes. Strengthening timely serological diagnosis, molecular surveillance, and integrated vector-control measures, particularly during the post-monsoon season, is essential for effective dengue prevention and control.
Keywords: Dengue virus, ELISA, DENV, Serotyping, Epidemiology
How to Cite?: Dr. Epibeni N. Humtsoe, Dr. Brajesh Joshi, "Evaluating the Efficacy of Serodiagnosis Technique of Dengue Virus Infection: An Epidemiological Study and Surveillance Analysis in Dimapur, Nagaland, India", Volume 15 Issue 10, October 2026, International Journal of Science and Research (IJSR), Pages: 371-374, https://www.ijsr.net/getabstract.php?paperid=SR261005130123, DOI: https://dx.doi.org/10.21275/SR261005130123