Comparative Evaluation of Ice Apple Water and Egg White with HBSS as a Storage Medium to Preserve Viability of Human Periodontal Ligament Fibroblasts: An in-Vitro Study

Dr. Savitha Sathyaprasad, Dr. Surabhi D, Ashwath AM

Abstract: Aim: To evaluate the efficacy of Ice apple water (IAW) and Egg white in preserving the PDL cell viability in comparison to Hanks balanced salt solution (HBSS) Methodology: 24 premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups, Group I (Ice Apple Water), Group II (Egg white), Group III(HBSS) 8 teeth in each group. After the teeth were extracted, the teeth were kept in their respective storage media for 60 minutes, PDL from middle & apical region were scraped using No.15 Blade and subsequently incubated in Falcon tube for 30 minutes in a mixture containing 2.5 mL of phosphate buffer and 0.5 mg of type I collagenase and falcon tubes were centrifuged for five minutes at 800 rpm. The cells were labeled with 0.4% trypan blue for determination of viability. Trypan Blue stains non-viable cells blue and viable cells appear colourless or pink. The number of viable PDL cells are counted under light microscope with hemocytometer at 40? magnification. Viabilty =100x [live cells/(live+dead cells)]. All the readings were noted and entered in the excel sheet. All data collected will be statistically analysed. Results: The assessment of cell viability with formula analysed using ANOVA test with p value <0.05 significant. The mean value of PDL cell viability for ice apple water was seen to be, 72.97?4.34 where as that of egg white was 74.80?2.89 while PDL cell viability in case of HBSS was the maximum with the mean value of 81.65?3.20 with p value 0.000181. The comparison of PDL cell viability was done between the 3 groups using post-hoc test with p value <0.05 as significant. PDL cell viability in case of HBSS was significantly more than that of egg white with mean difference, p value 0.0024659 PDL cell viability in case of HBSS was significantly more than that of ice apple water with mean difference, p value 0.0002145. PDL cell viability in case of ice apple water was similar to egg white with mean difference being, p value 0.5632173 Conclusion: Ice apple water and egg white because of good viability of cells, easier availability, and cost effectiveness can be used as a storage medium for avulsed tooth.

Keywords: Ice apple water, egg white, periodontal ligament cells, storage medium, cell viability

How to Cite?: Dr. Savitha Sathyaprasad, Dr. Surabhi D, Ashwath AM, "Comparative Evaluation of Ice Apple Water and Egg White with HBSS as a Storage Medium to Preserve Viability of Human Periodontal Ligament Fibroblasts: An in-Vitro Study", Volume 14 Issue 7, July 2025, International Journal of Science and Research (IJSR), Pages: 1846-1851, https://www.ijsr.net/getabstract.php?paperid=SR25725155558, DOI: https://dx.doi.org/10.21275/SR25725155558