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Research Paper | Dentistry | Volume 14 Issue 7, July 2025 | Pages: 1846 - 1851 | India
Comparative Evaluation of Ice Apple Water and Egg White with HBSS as a Storage Medium to Preserve Viability of Human Periodontal Ligament Fibroblasts: An in-Vitro Study
Abstract: Aim: To evaluate the efficacy of Ice apple water (IAW) and Egg white in preserving the PDL cell viability in comparison to Hanks balanced salt solution (HBSS) Methodology: 24 premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups, Group I (Ice Apple Water), Group II (Egg white), Group III(HBSS) 8 teeth in each group. After the teeth were extracted, the teeth were kept in their respective storage media for 60 minutes, PDL from middle & apical region were scraped using No.15 Blade and subsequently incubated in Falcon tube for 30 minutes in a mixture containing 2.5 mL of phosphate buffer and 0.5 mg of type I collagenase and falcon tubes were centrifuged for five minutes at 800 rpm. The cells were labeled with 0.4% trypan blue for determination of viability. Trypan Blue stains non-viable cells blue and viable cells appear colourless or pink. The number of viable PDL cells are counted under light microscope with hemocytometer at 40? magnification. Viabilty =100x [live cells/(live+dead cells)]. All the readings were noted and entered in the excel sheet. All data collected will be statistically analysed. Results: The assessment of cell viability with formula analysed using ANOVA test with p value
Keywords: Ice apple water, egg white, periodontal ligament cells, storage medium, cell viability
How to Cite?: Dr. Savitha Sathyaprasad, Dr. Surabhi D, Ashwath AM, "Comparative Evaluation of Ice Apple Water and Egg White with HBSS as a Storage Medium to Preserve Viability of Human Periodontal Ligament Fibroblasts: An in-Vitro Study", Volume 14 Issue 7, July 2025, International Journal of Science and Research (IJSR), Pages: 1846-1851, https://www.ijsr.net/getabstract.php?paperid=SR25725155558, DOI: https://dx.doi.org/10.21275/SR25725155558