GC-MS Analysis and In-Vitro Antioxidant Activity of Ethanolic Flower Extracts of Hibiscus Sabdariffa Linn and Moringa Oleifera from Bhagalpur Region

Priyanka Kumari, Manorama Singh, Bikal Kumar Gupta

Abstract: The escalating global incidence of oxidative stress-related pathologies has intensified the scientific pursuit of plant-derived therapeutics. This investigation evaluates the in vitro antioxidant efficacy of Hibiscus sabdariffa Linn and Moringa oleifera flowers, two phytochemically rich medicinal plants, with a focus on elucidating their bioactive constituents via Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Antioxidant capacities were quantified through DPPH, phosphomolybdate, Hydrogen peroxide and Ferric reducing power assays. The results revealed that both plant extracts exhibited robust antioxidant activity and their scavenging activities are in the order of H?O?>DPPH>Phosphomolybdate>FRAP. The chemical profiling of ethanolic flower extracts of Kudrum (Hibiscus sabdariffa Linn.) and drumstick tree (Moringa oleifera) using gas chromatography-mass spectrometry (GC-MS), identified a total of 45 compounds, with 24 from H. sabdariffa Linn. and 21 from M. oleifera. The ethanolic extract of H. sabdariffa Linn. flowers exhibited a high number of prominent peaks with key chemical constituents such as n-hexadecanoic acid, beta-Amyrin acetate, Ursolic aldehyde, .beta.-Amyrin, Tetradecanoic acid and Villosin. In contrast, the major components identified in M. oleifera flowers included Trimyristin, 4-oxide, (R)-, 1,1-diethoxyethane, Ethyl Oleate, Hexadecanoic acid and Methyl 9,10-octadecadienoate. These findings underscore the potential of Hibiscus sabdariffa Linn. and Moringa oleifera flowers as promising sources of natural antioxidants.

Keywords: GC-MS, Hibiscus sabdariffa Linn., Moringa oleifera, antioxidant, DPPH

How to Cite?: Priyanka Kumari, Manorama Singh, Bikal Kumar Gupta, "GC-MS Analysis and In-Vitro Antioxidant Activity of Ethanolic Flower Extracts of Hibiscus Sabdariffa Linn and Moringa Oleifera from Bhagalpur Region", Volume 14 Issue 7, July 2025, International Journal of Science and Research (IJSR), Pages: 1483-1492, https://www.ijsr.net/getabstract.php?paperid=SR25723171855, DOI: https://dx.doi.org/10.21275/SR25723171855