International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 4

India | Periodontology | Volume 14 Issue 7, July 2025 | Pages: 253 - 263


3D Cell Culture in Periodontics

Gopireddy Keerthi Reddy, Kranti K Reddy

Abstract: The complexity of periodontal tissues and the multifactorial nature of periodontal diseases necessitate advanced in vitro models that more accurately replicate in vivo conditions. This review critically evaluates the evolution, classification, and application of three-dimensional (3D) cell culture systems in periodontics. Compared to conventional two-dimensional (2D) cultures, 3D systems-comprising scaffold-based, scaffold-free, organ-on-a-chip, and microfluidic platforms-offer enhanced physiological relevance by simulating native extracellular matrix architecture, cellular heterogeneity, and microenvironmental cues. Scaffold-based models utilize biocompatible materials to support cell proliferation and differentiation, while scaffold-free techniques enable natural cell aggregation into spheroids and organoids. Organ-on-a-chip systems integrate dynamic flow conditions to mimic tissue interfaces and systemic interactions. These models facilitate improved understanding of periodontal pathogenesis, host?microbial interactions, inflammatory responses, and regenerative mechanisms. This review synthesizes evidences and emphasizing the utility of 3D cultures in drug screening, tissue engineering, and development of personalized therapies. Despite significant progress, challenges such as reproducibility, scalability, and cost must be addressed. Identification of research gaps and future directions underscores the transformative potential of 3D cell culture systems in advancing periodontal diagnostics and therapeutics. Further interdisciplinary efforts are warranted to integrate these platforms into clinical and translational research.

Keywords: 3D Cell cultures, Periodontal Regeneration, Scaffold-Based Models, Host?Pathogen Interactions, Tissue Engineering

How to Cite?: Gopireddy Keerthi Reddy, Kranti K Reddy, "3D Cell Culture in Periodontics", Volume 14 Issue 7, July 2025, International Journal of Science and Research (IJSR), Pages: 253-263, https://www.ijsr.net/getabstract.php?paperid=SR25626174510, DOI: https://dx.doi.org/10.21275/SR25626174510


Download Article PDF


Rate This Article!

Received Comments

No approved comments available.


Top