A Novel Differential Pulse Polarographic Method for the Electroanalytical Characterization of Thiamethoxam

L. M. Kashid

Abstract: A novel Differential Pulse Polarographic (DPP) method has been developed and validated for the electroanalytical characterization of thiamethoxam, a widely used neonicotinoid insecticide. Given the environmental persistence and increasing detection of thiamethoxam residues in water, food, and biological matrices, this study aims to establish a sensitive, cost-effective, and rapid electroanalytical alternative to conventional chromatographic and immunoassay techniques. Electrochemical behavior was investigated using a dropping mercury electrode in Britton-Robinson buffer (pH 3.0?11.0), with optimal response at pH 7.0. The method was optimized with a scan rate of 6 mV/s and pulse amplitude of 100 mV, yielding a well-defined cathodic peak at ?0.95 V. The reduction process was found to be pH-dependent, involving a two-step conversion of the nitroguanidine group. The proposed method showed excellent linearity in the 1.0?20.0 ?g/mL range (R? = 0.997), with a limit of detection (LOD) and quantification (LOQ) of 0.035 and 0.116 ?g/mL, respectively. The intra- and inter-day precision (%RSD <1.1%) and recovery rates (98.75?101.24%) confirmed the method?s accuracy and reproducibility. Furthermore, the technique demonstrated robustness under slight variations in pH, reinforcing its suitability for routine monitoring of thiamethoxam residues in environmental and agricultural samples. This study is among the first to detail the electrochemical reduction mechanism of thiamethoxam using DPP, offering a validated and practical tool for trace-level quantification.

Keywords: Differential Pulse Polarographic, thiamethoxam, Method Validation

How to Cite?: L. M. Kashid, "A Novel Differential Pulse Polarographic Method for the Electroanalytical Characterization of Thiamethoxam", Volume 14 Issue 5, May 2025, International Journal of Science and Research (IJSR), Pages: 574-579, https://www.ijsr.net/getabstract.php?paperid=SR25508231100, DOI: https://dx.doi.org/10.21275/SR25508231100