A Novel Differential Pulse Polarographic Method for the Electroanalytical Characterization of Thiamethoxam
Abstract: A novel Differential Pulse Polarographic (DPP) method has been developed and validated for the electroanalytical characterization of thiamethoxam, a widely used neonicotinoid insecticide. Given the environmental persistence and increasing detection of thiamethoxam residues in water, food, and biological matrices, this study aims to establish a sensitive, cost-effective, and rapid electroanalytical alternative to conventional chromatographic and immunoassay techniques. Electrochemical behavior was investigated using a dropping mercury electrode in Britton-Robinson buffer (pH 3.0?11.0), with optimal response at pH 7.0. The method was optimized with a scan rate of 6 mV/s and pulse amplitude of 100 mV, yielding a well-defined cathodic peak at ?0.95 V. The reduction process was found to be pH-dependent, involving a two-step conversion of the nitroguanidine group. The proposed method showed excellent linearity in the 1.0?20.0 ?g/mL range (R? = 0.997), with a limit of detection (LOD) and quantification (LOQ) of 0.035 and 0.116 ?g/mL, respectively. The intra- and inter-day precision (%RSD
Keywords: Differential Pulse Polarographic, thiamethoxam, Method Validation
How to Cite?: L. M. Kashid, "A Novel Differential Pulse Polarographic Method for the Electroanalytical Characterization of Thiamethoxam", Volume 14 Issue 5, May 2025, International Journal of Science and Research (IJSR), Pages: 574-579, https://www.ijsr.net/getabstract.php?paperid=SR25508231100, DOI: https://dx.doi.org/10.21275/SR25508231100